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cellenone robot  (Cellenion)

 
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    Cellenion cellenone robot
    Cellenone Robot, supplied by Cellenion, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellenone+robot/cellenone+robot/pm39820751-266-35-32
    Average 90 stars, based on 1 article reviews
    cellenone robot - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Cellenone:

    Article Title: Challenging the Astral mass analyzer to quantify up to 5,300 proteins per single cell at unseen accuracy to uncover cellular heterogeneity.
    Article Snippet: .. In brief, 300 nl of master mix (0.2% DDM, 10 ng μl–1 Trypsin Gold and 100 mM TEAB) was dispensed into each well of a LF48 proteoCHIP or an EVO 96 proteoCHIP (Cellenion) using the cellenONE robot. .. The LF48 chip was prefilled with 2 μl of hexadecane oil (H6703-100ML, Sigma-Aldrich) per well.

    Article Title: Robust and Easy-to-Use One-Pot Workflow for Label-Free Single-Cell Proteomics
    Article Snippet: .. When using the cellenONE robot (Cellenion), diameter and elongation are the relevant parameters. ..



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    the cellenone® robot  (Cellenion)
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    Individual A549 cells were prepared in a 384 well plate using the <t>cellenONE</t> in predefined size groups of 15-20, 20-25 or 25-30 µm diameter. Digested cells were analyzed at 50 SPD using the previously optimized settings for LC and MS on the Orbitrap Astral MS. A : Circles indicate identified protein groups (PG) at 1% FDR of each individual cell, bars indicate their means, while error bars indicate standard deviations. The search was performed library free in method evaluation mode, or with matching, or against a tailored library created from 3 replicates of 20x or 40x cell,all samples prepared and measured in the exact same way. Blanks were processed in the same 384 well plate and contained all reagents and buffers but no cell. B : PCA and C : UMAP based on protein quantities with each dot representing a cell and colors reflecting the actual cell size as determined by the camera system of the cellenONE.
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    Image Search Results


    Individual A549 cells were prepared in a 384 well plate using the cellenONE in predefined size groups of 15-20, 20-25 or 25-30 µm diameter. Digested cells were analyzed at 50 SPD using the previously optimized settings for LC and MS on the Orbitrap Astral MS. A : Circles indicate identified protein groups (PG) at 1% FDR of each individual cell, bars indicate their means, while error bars indicate standard deviations. The search was performed library free in method evaluation mode, or with matching, or against a tailored library created from 3 replicates of 20x or 40x cell,all samples prepared and measured in the exact same way. Blanks were processed in the same 384 well plate and contained all reagents and buffers but no cell. B : PCA and C : UMAP based on protein quantities with each dot representing a cell and colors reflecting the actual cell size as determined by the camera system of the cellenONE.

    Journal: bioRxiv

    Article Title: Challenging the Astral™ mass analyzer — going beyond 5200 proteins per single-cell at unseen quantitative accuracy to study cellular heterogeneity

    doi: 10.1101/2024.02.01.578358

    Figure Lengend Snippet: Individual A549 cells were prepared in a 384 well plate using the cellenONE in predefined size groups of 15-20, 20-25 or 25-30 µm diameter. Digested cells were analyzed at 50 SPD using the previously optimized settings for LC and MS on the Orbitrap Astral MS. A : Circles indicate identified protein groups (PG) at 1% FDR of each individual cell, bars indicate their means, while error bars indicate standard deviations. The search was performed library free in method evaluation mode, or with matching, or against a tailored library created from 3 replicates of 20x or 40x cell,all samples prepared and measured in the exact same way. Blanks were processed in the same 384 well plate and contained all reagents and buffers but no cell. B : PCA and C : UMAP based on protein quantities with each dot representing a cell and colors reflecting the actual cell size as determined by the camera system of the cellenONE.

    Article Snippet: In brief, 300 nL of mastermix (0.2% DDM, 10 ng/µL Trypsin Gold, 100 mM TEAB) were dispensed into each well of the LF48 proteoCHIP® or the Evo96 ProteoCHIP® (Cellenion, Lyon, France) using the cellenONE® robot.

    Techniques: